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. 2021 Sep 12;10(9):900. doi: 10.3390/biology10090900

Figure 4.

Figure 4

Melting curves at ~180 nm of DsrAcore and rpoSreg complexes annealed without protein (red), with full-length Hfq (green), with Hfq-CTR (blue), and heat pre-hybrided DsrAcore:rpoSreg without protein (black), respectively. For a better comparison of various sigmoids, they were normalized between 0% and 100%. The corresponding Tm were 36.6 ± 0.8 °C, 37.0 ± 0.9 °C, 33.6 ± 2.2 °C, and 39.9 ± 0.4 °C.