Table 1.
Melting point (Tm) of RNA measured at 180 nm. DsrAcore + rpoSreg means not prehybrided, DsrAcore:rpoSreg means prehybrided by heat and cooling (see methods).
| Sample | Tm at ~180 nm (°C) |
|---|---|
| DsrAcore + rpoSreg | 33.6 ± 2.2 |
| DsrAcore + rpoSreg + CTR | 36.6 ± 0.8 |
| DsrAcore + rpoSreg + Hfq | 37.0 ± 0.9 |
| DsrAcore:rpoSreg | 39.9 ± 0.4 |
| DsrAcore:rpoSreg + CTR | 44.2 ± 0.7 |
| DsrAcore | 42.1 ± 0.3 |
| DsrAcore + CTR | 45.5 ± 0.8 |
| DsrAcoremut | 49.1 ± 1.2 |
| DsrAcoremut + CTR | 50.3 ± 0.9 |
| rpoSrbs + rpoSreg | 29.5 ± 2.2 |
| rpoSrbs + rpoSreg + CTR | 31.6 ± 1.7 |