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. 2021 Sep 14;22(18):9885. doi: 10.3390/ijms22189885

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of papain on adipocyte differentiation in MDI–treated 3T3–L1 preadipocytes. (a) Cell viability of papain in 3T3–L1 preadipocytes. *** p < 0.001 vs. non-treated group. (b) Oil red O staining 8 days after induction. (c) Quantitative analysis of Oil Red O staining. Oil Red O stained cells were extracted with isopropyl alcohol and the absorbance at 520 nm was measured. (d) Macroscopic shots of oil red O staining are shown. The values are given as the mean ± SD ^### p < 0.001 vs. non-treated group; *** p < 0.001 vs. MDI-treated group.