Figure 7.

(a) Plasma concentration versus time profile (two-compartmental model) (mean ± SEM, n = 4) of the C5aR1 peptide antagonist administered by bolus intravenous (IV) injection at a dose of 1 mg/kg either as free compound (black) or in the form of lipid-shell PLGA nanoparticles (pink). (b) Biodistribution (mean ± SEM, n = 4) of peptide released from nanoparticles in different organs (brain, spinal cord, liver, lung, kidney, spleen) (ng/g). Tissues were extracted on day one and day five after a single IV injection of 1 mg/kg either as free peptide (black on day one due to the short half-life of this peptide based on our previous PK studies for the free peptide) or in the form of nanoparticles (pink on day five), respectively. Statistical analysis was conducted by two-way ANOVA, followed by Sidak’s test for multiple comparisons (**** p < 0.0001).