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. 2021 Sep 6;10(9):2336. doi: 10.3390/cells10092336

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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dGOLPH3 interacts with vesicle trafficking and cytoskeleton proteins. (a) Protein extracts from testes expressing either GFP or the indicated YFP-tagged Rab protein were immunoprecipitated with GFP traps and blotted to detect either GFP/YFP or GOLPH3. 4% of the total lysates and one third of IP were loaded and probed with the indicated antibodies. (b) Protein extracts from Drosophila testes expressing GFP (control) and GFP tagged Rac1, Rac2 and Sec22 were immunoprecipitated with GFP trap and blotted to detects either GFP or GOLPH3. 4% of the total lysates and one third of IP were loaded and probed with the indicated antibodies. Molecular masses in (a,b) are expressed in kilodaltons.