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dGOLPH3 interacts with endocytic trafficking proteins. GST (control) and recombinant GST-GOLPH3 proteins, immobilized on Gluthatione-Sepharose beads were incubated with testis protein extracts from Oregon-R males. Ponceau staining is shown as a loading control. 2% of the input and 25% of the pull-down were loaded and probed for SH3PX1 (a) and Shibire (b). Molecular masses are expressed in kilodaltons.
