Table 1.
Selected proteins interacting with dGOLPH3, categorized by function. The full list of hits in the dGOLPH3 interactome is shown in supplementary Table S1. Protein identifications were verified by manual inspection of MS/MS spectra.
| UniProtKD Entry 1 | Drosophila Annotation Symbol 2 | Drosophila Symbol 3 | PEP 4 |
|---|---|---|---|
| Golgi vesicle transport—GO: 0048193 | |||
| Q9W0B8 | CG7961 | αCOP | 3.71 × 10−115 |
| P45437 | CG6223 | βCOP | 2.64 × 10−71 |
| O62621 | CG6699 | β’COP | 6.21 × 10−70 |
| Q8I0G5 | CG1528 | γCOP | 6.38 × 10−40 |
| Q9W555 | CG14813 | δCOP | 1.35 × 10−28 |
| A1Z7J7 | CG8266 | Sec31 | 2.17× 10−15 |
| M9PGI6 | CG7359 | Sec22 | 1.47 × 10−12 |
| Q9VLS7 | CG8552 | PAPLA1 | 1.59 × 10−10 |
| Q9VQ94 | CG10882 | Sec24CD | 3.34 × 10−10 |
| Q9VNF8 | CG1250 | Sec23 | 1.29 × 10−09 |
| Q24179 | CG3539 | Slh | 1.88 × 10−05 |
| Q9Y0Y5 | CG9543 | εCOP | 1.83 × 10−04 |
| Q9VAD6 | CG31040 | Cog7 | 4.91 × 10−03 |
| Rab protein signal transduction—GO: 0032482 | |||
| O18332 | CG3320 | Rab1 | 3.74 × 10−69 |
| A1Z7S3 | CG8024 | Rab32 | 5.99 × 10−23 |
| Q9V3I2 | CG3664 | Rab5 | 2.60 × 10−22 |
| O18338 | CG8287 | Rab8 | 5.90 × 10−06 |
| O15971 | CG17060 | Rab10 | 5.90 × 10−06 |
| Q86BK8 | CG4212 | Rab14 | 1.63 × 10−05 |
| O18335 | CG5771 | Rab11 | 8.83 × 10−05 |
| Transport—GO: 006810 | |||
| P29742 | CG9012 | Chc | 1.36 × 10−43 |
| Q9VS57 | CG8583 | Sec63 | 1.58 × 10−43 |
| Q9V3D9 | CG4659 | Srp54k | 1.90 × 10−22 |
| P27619 | CG18102 | Shi | 1.23 × 10−10 |
| Q9VEX2 | CG6963 | Gish | 1.21 × 10−05 |
| Q9NCC3 | CG6757 | SH3PX1 | 3.91 × 10−05 |
| Protein glycosylation—GO: 0006486 | |||
| Q7K110 | CG6370 | OstΔ | 5.06 × 10−11 |
| Q09332 | CG6850 | Uggt | 9.90 × 10−10 |
| Q9VP06 | CG11306 | Alg11 | 6.42 × 10−08 |
| Q9XZ08 | CG15110 | Botv | 9.17 × 10−06 |
| Q6WV17 | CG31651 | Pgant5 | 1.87 × 10−05 |
| Q9VTZ6 | CG10688 | Pmm2 | 3.61 × 10−04 |
| Q8MV48 | CG6394 | Pgant7 | 1.17 × 10−02 |
| Lipid metabolic process—GO: 0006629 | |||
| Q9W0I6 | CG9128 | Sac1 | 1.43 × 10−49 |
| Q9VXH3 | CG4200 | Sl | 1.04 × 10−10 |
| Q9VL10 | CG31873 | Mulk | 4.69 × 10−10 |
| Q9VGM0 | CG6790 | CG6790 | 4.19 × 10−09 |
| Q9VZL3 | CG10849 | Sc2 | 1.13 × 10−08 |
| Q9VGL9 | CG5342 | CG5342 | 2.21 × 10−08 |
| Q9VCV7 | CG4907 | CG4907 | 3.48 × 10−07 |
| Q9V7Y2 | CG8946 | Sply | 2.20 × 10−06 |
| Q9VCY7 | CG6921 | Bond | 5.56 × 10−04 |
| Q9W3G0 | CG11190 | PIG-T | 3.44 × 10−03 |
| Golgi organization—GO: 0007030 | |||
| Q7KN62 | CG2331 | TER94 | 5.55 × 10−45 |
| Q7KNA0 | CG8230 | CG8230 | 4.24 × 10−16 |
| Q9V3A8 | CG6822 | ergic53 | 1.23 × 10−05 |
| Cell cycle—GO: 0007049 | |||
| P36179 | CG17291 | Pp2A-29B | 2.55 × 10−11 |
| Q9VAK1 | CG1906 | Alph | 2.56 × 10−05 |
| P36872 | CG6235 | Tws | 2.18 × 10−03 |
| P40423 | CG3595 | Sqh | 4.19 × 10−03 |
| Signaling—GO: 0023052 | |||
| P29310 | CG17870 | 14-3-3ζ | 1.36 × 10−57 |
| P40792 | CG2248 | Rac1 | 1.01 × 10−41 |
| P48554 | CG8556 | Rac2 | 1.01 × 10−41 |
| Q9VGS2 | CG4800 | Tctp | 1.71 × 10−06 |
| O61444 | CG9738 | Mkk4 | 1.43 × 10-04 |
| Q9W328 | CG3004 | Lst8 | 8.71 × 10−04 |
| P40793 | CG12530 | Cdc42 | 1.72 × 10−03 |
| Cilium assembly—GO: 0060271 | |||
| Q8INT5 | CG31623 | Dtr | 2.96 × 10−15 |
| Q9V3M9 | CG4767 | Tektin-A | 4.85 × 10−13 |
| Q9W1D3 | CG3121 | Rsph4a | 1.12 × 10−08 |
| O96860 | CG5450 | Cdlc2 | 1.58 × 10−05 |
| Q24117 | CG6998 | Ctp | 1.58 × 10−05 |
| Q960Z0 | CG1453 | Klp10A | 1.61 × 10-05 |
| Q8T3V7 | CG31803 | Rsph9 | 1.72 × 10−05 |
| Q9W1U1 | CG12192 | Klp59D | 1.92 × 10−05 |
| Q9VQV7 | CG3980 | Cep97 | 3.60 × 10−05 |
| Q9VJY4 | CG10859 | CG10859 | 2.45 × 10−04 |
| Q8MSJ9 | CG9313 | CG9313 | 1.12 × 10−03 |
| Nervous system process—GO: 0050877 | |||
| Q9NFU0 | CG6203 | Fmr1 | 9.29 × 10−41 |
| Q8SWR8 | CG5166 | Atx2 | 8.52 × 10−11 |
1 UniProtKD entry; unique and stable entry identifier. 2 Drosophila annotation symbol; Current FlyBase annotation identifier of the gene. CG, prefix for protein-coding genes. 3 Drosophila symbol; Approved Drosophila gene/protein symbol. 4 PEP; Posterior Error Probability of the identification. This value essentially operates as a p-value and represents the probability that the observed peptide spectrum match (PSM) is incorrect (a smaller value is more significant).