Figure 2.

Immunofluorescence images demonstrating the co-culture and triculture of the BBB on a chip. iBMECs cultured on all surfaces of the blood channel, and primary human brain pericytes and astrocytes on the apical brain channel. (A–C) Different magnifications of iBMECs stained with ZO-1 (red); iBMECs form a tight barrier on the blood side; astrocytes were stained with GFAP (green). (D,G) 3D structure of the co-culture. (E) Co-culture staining of primary human astrocytes (GFAP, green) and primary human pericytes (α-SMA, yellow) in the brain channel. (F) Triculture staining of the BBB on a chip (red, iBMECs-ZO-1; green, astrocytes (GFAP); yellow, pericytes (α-SMA)).