Table 2.

Cryo-TEM analysis of NPCE-derived EVs before and after electroporation.

	Untreated NPCE EVs	Condition 1	Condition 2	Condition 3	Condition 4
Mean of Normal Shaped EVs (%)	91.31	76.02	64.16	65.86	72.86
Mean of Damaged EVs (%)	8.69	23.98	35.84	34.14	27.14

Cryo-TEM analysis for the evaluation of normal or damaged-shaped EVs as a percentage of the total particle population in each image. A total of 91.31% of normal shaped EVs were observed for untreated NPCE EVs, 76.02%, 64.16%, 65.86%, and 72.86% of electroporated NPCE EVs in various conditions were determined. A minimum of 50 images for each group were taken.