Table 3.

qRT-PCR analysis of electroporated TM cells for the assessment of SMAD7 gene silencing.

Treatments	Mean Ct Value (SMAD7)	Relative mRNA Levels (GAPDH)	Relative mRNA Levels
Untreated TM Cells	24.041	23.223	1 ± 0.454
Electroporated TM Cells	25.128	25.976	3.146 ± 1.315
Non-targeting siRNA + TM Cells	24.038	23.941	1.562 ± 0.102
SMAD7 siRNA + TM Cells	26.470	24.575	0.47 ± 0.015

The expression of SMAD7 in TM cells was measured after electroporation of siRNA. Untreated TM cells, electroporated TM cells, and non-targeting siRNA loaded to TM cells were used as negative controls. Mean Ct (cycle threshold) values of TM cells loaded with siRNA against SMAD7 revealed a reduction of 2.5 cycles in SMAD7 expression compared to untreated TM cells.