Table 4.
Human Target Gene (25 nmol) |
Primers | |
---|---|---|
Forward | Reverse | |
SMAD7 | 5′-CCA ACT GCA GAC TGT CCA GA-3′ | 5′-TTC TCC TCC CAG TAT GCC AC-3′ |
TGFβ2 | 5′-AAG AAG CGT GCT TTG GAT GCG G-3′ | 5′-ATG CTC CAG CAC AGA AGT TGG C-3′ |
GSK-3β | 5′-CCG ACT AAC ACC ACT GGA AGC T -3′ | 5′-AGG ATG GTA GCC AGA GGT GGA T-3′ |
β-Catenin | 5′-CAC AAG CAG AGT GCT GAA GGT G-3′ | 5′-GAT TCC TGA GAG TCC AAA GAC AG-3′ |
GAPDH | 5′-GCA CCG TCA AGG CTG AGA AC-3′ | 5′-GGA TCT CGC TCC TGG AAG ATG-3′ |