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. 2021 Sep 18;22(18):10080. doi: 10.3390/ijms221810080

Figure 4.

Figure 4

ZmWRKY79 overexpression promoted stomatal closure and ABA synthesis. (A) Stomata of ZmWRKY79-OEs (OE-8 and OE-10) and WT Arabidopsis plants with 5 μM ABA treatment. The untreated samples were used as the control. Bars mean 20 μm. (B) Stomatal aperture of OE-8, OE-10, and WT with or without ABA treatment. (C) Water loss rates of OE-8, OE-10, and WT-detached leaves at room temperature. (D–E) Accumulation of ABA (D) and JA-Ile (E) in OE-8 and WT with 20% PEG6000 treatment. Asterisks indicate significant difference compared to WT (Student’s t-test, * p < 0.05, ** p < 0.01). Different lowercase letters indicate significant difference (LSD test, p < 0.05). Error bars indicate SE (n = 3).