Figure 1.

The target site for knockout Period gene (Per) and the mutant screening in B. mori. (A) Gene structure and the TALEN knockout sequences (302G...351T) designed for the transcription start site (300ATG) located on the third exon of Per. (B) Mutant screening efficiency and the 3 types of Per gene mutations screened. (C) Sequencing peak map of ∆Per-3 mutant. (D,E) Verification of Per knockout effect on mRNA level and protein level respectively. The material is the newly hatched larvae (n = 3 batches, 200–300 larvae per batch).