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Antiubiquitin antibody recognizes high-molecular-weight forms of Rpb1 from 4-NQO-treated cells. Yeast cells were treated with 4-NQO at 4 μg/ml for 30 min, and whole-cell extracts were prepared. Rpb1 was immunoprecipitated (IP) in duplicate from each sample with anti-CTD antibody. The immunoprecipitates were then analyzed by SDS-PAGE followed by immunoblotting with either anti-CTD (αRpb1) or antiubiquitin (αub.) antibody.
