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qPCR validation of miRNA expression: (A) miR-181a and other miR-181 family members (181b-5p, miR-181c-5p, and miR-181d-5p) and (B) miR-320a in LCLs. SIB = siblings; CNT = controls; ASD = autism spectrum disorder; data are means ± SEM of n = 9–10 samples in each category. Error bars represent standard error of the mean. miRNA expression was normalized to RNU6 for each group. * p ≤ 0.05.; ** p ≤ 0.01.
