Figure 2.

Dynasore inhibits the development of preAC. (A) Cells were plated on coverslips, infected with Δm138-MCMV, and at 4 hpi treated with dynasore (80 µM) or left untreated. At 6, 14, and 48 hpi, the cells were fixed, permeabilized, and stained against Rab10 (EE/ERC marker; red fluorescence), GM130 (cis-Golgi; green fluorescence), and IE1 (a nuclear marker of infection; blue fluorescence). In mock-infected cells, the blue color represents the DAPI nuclear staining. Scale bar is equal to 10 µm in smaller and 5 µm in larger magnifications. Representative images of three independent experiments are shown. (B) Quantification of Rab10 accumulation and the Golgi patterns in immunofluorescence experiments. The left panel presents the percentage of infected cells with juxtanuclear accumulation of Rab10 (inner preAC), whereas the right panel presents ratios of different Golgi patterns visualized by GM130. Data represent the mean values, and error bars show standard deviations. The significance to the untreated samples of the same kinetics was determined by the Student’s t-test (*** p < 0.001). (C) Schematic presentation of Golgi patterns.