Figure 3.

The effect of dynamin inhibitors on the establishment of the preAC and development of the AC. Δm138-MCMV infected cells were treated with dynasore (80 µM), Dyngo-4a (200 µM), MiTMAB (20 µM), and Dynole-34-2 (10 µM) at 4 hpi, and incubated in the presence of inhibitors up to 16 and 40 hpi. (A) Double and triple immunofluorescence images of 16 and 40 h infected cells, respectively, stained against IE1 (green), Rab10 (red), and M55/gB (blue). Asterisks point to M55 in the AC and arrowheads to M55 at the cell surface. The scale bar is equal to 10 µm. Representative images of three independent experiments are shown. (B) Quantification of the impact of inhibitors on establishing the preAC indicated by Rab10 accumulation (left panel) and M55 loading into the oAC (right panel). Results on graphs are presented as normalized to control [(%Rab10_Inh./%Rab10_Ø) or (%M55-AC_Inh./%M55-AC_Ø)]. Only IE1⁺ cells were analyzed. The data represent the mean ± SD from 3–4 independent experiments. The significance of differences to untreated samples from the same kinetics was determined using the Student’s t-test (*** p < 0.001, ** p < 0.01, * p < 0.05).