Figure 4.

The effect of dynasore on the pericentriolar accumulation of EE in MCMV infected Balb3T3 cells. Cells were plated on coverslips, infected with Δm138-MCMV, and treated with dynasore (Dy, 80µM) at 4 hpi or left untreated (Ø). At 6 and 14 hpi, cells were proceeded for immunofluorescence. (A) Immunofluorescence images of EE markers (red) and IE1 (green) at 6 and 14 hpi. (B) Percentage of (IE1⁺) cells demonstrating pericentriolar accumulation of EE markers. (C) The schematic presentation of localization of EE vesicles in preAC. Mean values from 2–4 independent experiments are plotted, error bars show standard deviations. The significance of differences to untreated sample of the same kinetics was determined using the Student’s t-test (*** p < 0.001, ** p < 0.01, * p < 0.05).