Figure 9.

The effect of dynasore on the expression of late MCMV proteins and virion production. Δm138-MCMV infected cells were treated with dynasore (80 μM) at 4–48 hpi or 4–14 hpi. At indicated times, the samples were analyzed by Western blot (A–D), flow cytometry (E), and plaque assay (F). (A–C) Representative Western blots of M74 (A), M25 (B), and M55 (C) expression in the 4–48 hpi dynasore-treated cells. (D) Western blot of M55 expression in 4–14 hpi dynasore-treated cells. In all Western blot experiments, the β-actin, a cellular loading control, was visualized either sequentially or simultaneously at the same membrane and used to normalize the signal in densitometric analysis. (E) Flow cytometric quantification of M55 expression in untreated (Ø) and 4–48 or 4–14 hpi dynasore-treated cells. The data present the relative percentage of M55-positive cells (mean ± SD). (F) Quantification of extracellular virions in supernatants at 48 and 72 hpi of untreated and dynasore-treated (4–48, 4–72, and 4–14 hpi) cells. The data represent the mean values of four independent experiments, and error bars show standard deviations. The significance of differences was determined using the Student’s t-test (*** p < 0.001, ** p < 0.01).