Table 1.
Quantification of pericentriolar accumulation of Rab10 and the Golgi reorganization in untreated and dynasore (Dy)-treated MCMV-infected cells.
| Pericentriolar Rab10 (%) * | Golgi Patterns (%) * | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Aa | Ab | B | C | |||||||
| Ø | Dy | Ø | Dy | Ø | Dy | Ø | Dy | Ø | Dy | |
| Mock | 0 | - | 67.8 ± 4.2 | - | 27.2 ± 1.4 | - | 4.4 ± 2.3 | - | 0.4 ± 0.7 | - |
| 6 hpi | 33.3 ± 2.9 | 0.6 ± 1.1 | 7.3 ± 1.8 | 15.4 ± 4.9 | 25.9 ± 10.5 | 56.1 ± 7.2 | 42.7 ± 10.7 | 25.9 ± 9.0 | 23.9 ± 6.3 | 2.6 ± 2.9 |
| 14 hpi | 72.1 ± 3.6 | 5.4 ± 2.2 | 1.9 ± 0.7 | 0.9 ± 0.5 | 9.4 ± 3.9 | 58.7 ± 2.9 | 14.2 ± 4.2 | 35.3 ± 1.6 | 74.4 ± 7.5 | 5.0 ± 3.7 |
| 48 hpi | 78.9 ± 8.5 | 10.7 ± 5.8 | 0 | 2.7 ± 1.3 | 2.8 ± 0.7 | 70.5 ± 3.7 | 7.1 ± 4.2 | 22.9 ± 6.2 | 90.0 ± 5.0 | 3.8 ± 3.7 |
* mean ± standard deviation. Δm138-MCMV infected Balb3T3 cells were treated with dynasore (80 µM) at 4 hpi (Dy) or left untreated (Ø). The cells were processed for immunofluorescence labeling at 6, 14, and 48 hpi. The percentage of cells in three independent experiments with the pericentriolar accumulation of Rab10 and different Golgi patterns was quantified as explained in Section 2.