Table 3.
Quantification of dynasore effect on the recruitment of EE/ERC/Golgi markers in preAC of MCMV infected Balb3T3 cells.
| Marker of Cellular Compartment | The Ratio of Infected Cells (%) with the Accumulated Marker in Pericentriolar Membranes * | |||
|---|---|---|---|---|
| 6 hpi | 14 hpi | |||
| Untreated | Dynasore | Untreated | Dynasore | |
| Early Endosomes | ||||
| Rab5 | 50.1 ± 4.5 | 10.7 ± 1.9 | 71.5 ± 7.6 | 29.7 ± 1.6 |
| Vps34 | 28.0 ± 3.5 | 4.0 ± 0.2 | 63.8 ± 4.1 | 8.3 ± 3.2 |
| EEA1 | 20.7 ± 4.1 | 2.9 ± 0.6 | 62.4 ± 7.5 | 4.0 ± 1.3 |
| Hrs | 37.2 ± 1.8 | 4.0 ± 0.1 | 67.3 ± 3.6 | 5.6 ± 2.9 |
| Early Endosomes to Endosomal Recycling Compartment | ||||
| Rab10 | 33.3 ± 2.9 | 0.6 ± 1.1 | 72.1 ± 3.6 | 5.4 ± 2.2 |
| Endosomal Recycling Compartment | ||||
| Rab11 | 64.1 ± 4.3 | 15.9 ± 3.9 | 78.6 ± 3.7 | 51.3 ± 7.5 |
| Arf6 | 1.0 ± 0.5 | 0 | 67.81 ± 5.8 | 7.6 ± 2.7 |
| Golgi Compartments | ||||
| Grasp65 (ERGIC-cis-Golgi) | 28.0 ± 2.0 | 6.0 ± 0.5 | 71.8 ± 5.1 | 8.7 ± 2.8 |
| GM130 (cis-Golgi) | 23.9 ± 13.3 | 2.6 ± 2.9 | 74.4 ± 7.5 | 5.0 ± 3.7 |
| Golgin97 (trans-Golgi) | 29.1 ± 2.0 | 5.4 ± 3.1 | 69.2 ± 6.5 | 7.0 ± 0.6 |
| Grasp65 (ERGIC-cis-Golgi) | 28.0 ± 2.0 | 6.0 ± 0.5 | 71.8 ± 5.1 | 8.7 ± 2.8 |
| Late Endosomes | ||||
| Lamp1 | 0 | 0 | 0 | 0 |
* mean ± standard deviation. Balb 3T3 cells were infected with Δm138-MCMV, treated with dynasore (80 µM), or left untreated, and proceeded for immunofluorescence. Quantification was performed as explained in Section 2. The data represent mean ± SD from 2–4 independent experiments.