Table 1.
Sequences, genomic positions, and amplicon sizes of the primers used in qRT-PCR for the target and reference genes. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RPL13a, ribosomal protein L13a; SDHA, succinate dehydrogenase complex, subunit A; Twist1, twist basic helix-loop-helix transcription factor 1; Snail1, snail family zinc finger 1; Snail2, snail family zinc finger 2; Zeb1, zinc finger E-box binding homeobox 1; Zeb2, zinc finger E-box-binding homeobox 2.
| Gene | Primer Sequence | Exons | Amplicon Length |
|---|---|---|---|
| GAPDH | F: 5′-ATCATCAGCAATGCCTCCTG-3′ R: 5′-GGCCATCCACAGTCTTCTG-3′ |
6 to 7 8 |
136 bp |
| RPL13a | F: 5′-ACCGTGCGAGGTATGCTG-3′ R: 5′-TAGGCTTCAGACGCACGAC-3′ |
4 to 5 6 |
148 bp |
| SDHA | F: 5′-GCATAAGAACATCGGAACTGC-3′ R: 5′-GGTCGAACGTCTTCAGGTG-3′ |
12 13 |
147 bp |
| Twist1 | F: 5′-ATGTCATTGTTTCCAGAGAAGG-3′ R: 5′-CCACGCCCTGTTTCTTTG-3′ |
16 17 |
137 bp |
| Snail1 | F: 5′-ACCCACACTGGCGAGAAG-3′ R: 5′-CAGGGACATTCGGGAGAAG-3′ |
19 20 |
150 bp |
| Snail2 | F: 5′-GGTTGCTTCAAGGACACATTAG-3′ R: 5′-TGGAGAAGGTTTTGGAGCAG-3′ |
12 13 |
162 bp |
| Zeb1 | F: 5′-ACCACCCTTGAAAGTGATCC-3′ R: 5′-CTGATTCTACACCGCCCAAA-3′ |
2 3 |
115 bp |
| Zeb2 | F: 5′-CCCTTCTGCGACATAAATACG-3′ R: 5′-CGAGTGAAGCCTTGAGTGC-3′ |
1 2 |
113 bp |
| E-cadherin | F:5′-CATTCTGGGGATTCTTGGAG-3′ R: 5′-CCGCCTCCTTCTTCATCATA-3′ |
1 2 |
156 bp |
| Vimentin | F:5′-GAGAGAGGAAGCCGAAAACAC-3′ R: 5′-TCCACTTTGCGTTCAAGGTC-3′ |
1 2 |
90 bp |