FIG. 6.

Clk and ClkR modulate SV40 pre-mRNA alternative splicing. (A) Effect of Clk and ClkR on SV40 pre-mRNA splicing in nuclear extract. Lane 1, nuclear extract alone; lanes 2 to 4 and 5 to 7, increasing amounts (50, 100, and 200 ng) of Clk and ClkR, respectively. (B) Effect of Clk and ClkR on SV40 pre-mRNA splicing in S100 extracts complemented with ecASF (750 ng). Lane 1, ecASF only; lanes 2 to 4 and 5 to 7, increasing amounts (50, 100, and 200 ng) of Clk and ClkR, respectively. Positions of pre-mRNA, spliced products, and intermediates are shown. A schematic of the pre-mRNA is shown at the bottom.