Table 4.
Phenotypic detection of carbapenemase production in carbapenem-resistant Gram-negative bacterial isolates.
| Tested Isolates | Blue-Carba Test (n = 65) [26] |
Modified Carbapenem Inactivation Method (mCIM) (n = 57) [25] |
EDTA-Modified Carbapenem Inactivation Method (eCIM) (n = 29) [25] |
|||
|---|---|---|---|---|---|---|
| No. of CPs/Total No. of Tested Isolates | % | No. of CPs/Total No. of Tested Isolates | % | No. of MBL Producers/Total No. of Tested Isolates | % | |
| Klebsiella spp. | 22/28 | 78.6 | 23/28 | 82.1 | 12/23 | 52.2 |
| P. aeruginosa | 7/19 | 36.8 | 2/19 | 10.5 | ND | ND |
| A. baumannii | 1/8 | 12.5 | ND | ND | ND | ND |
| E. coli | 3/6 | 50 | 4/6 | 66.7 | 3/4 | 75 |
| Proteus mirabilis | 2/2 | 100 | 1/2 | 50 | 0/1 | 0 |
| Enterobacter cloacae | 1/2 | 50 | 1/2 | 50 | 1/1 | 100 |
Notes: Blue-Carba test is a phenotypic test for the detection of carbapenemase production in Enterobacterales, Pseudomonas spp., and Acinetobacter spp. Modified carbapenem inactivation method (mCIM) test is a phenotypic test for the detection of carbapenemase production in Enterobacterales and Pseudomonas spp., but not Acinetobacter spp. EDTA-modified carbapenem inactivation method (eCIM) is carried out only for Enterobacterales that give a positive result in mCIM. CPs, carbapenemase-producers; MBL, metallo-β-lactamase; ND, not determined.