Table 6.
Phenotypic and genotypic characteristics of the transformed plasmids.
| Parental Strain | Transformant Code | Plasmid Code | Acquired Transformant Phenotype | Plasmid Genotype | Plasmid Size in Gel | |
|---|---|---|---|---|---|---|
| 24.SK | TS24.SK | pKPT24 | Imipenem (10 µg) | I | blaOXA-48/blaCTX-M | 2 plasmid bands: Large plasmid: >10 kb Small plasmid: ≈1700 bp |
| Meropenem (10 µg) | R | |||||
| Ertapenem (10 µg) | R | |||||
| Ampicillin/sulbactam (20 µg) | R | |||||
| Cefoxitin (30 µg) | R | |||||
| Ceftazidime (30 µg) | R | |||||
| Ceftriaxone (30 µg) | I | |||||
| Cefepime (30 µg) | SDD | |||||
| Ciprofloxacin (5 µg) | R | |||||
| Levofloxacin (5 µg) | R | |||||
| 37.AK | TS37.AK | pKPT37 | Imipenem (10 µg) | I | bla OXA-48 | 2 plasmid bands: Large plasmid: >10 kb Small plasmid: >10 kb |
| Ampicillin/sulbactam (20 µg) | R | |||||
| Cefoxitin (30 µg) | R | |||||
| Ceftazidime (30 µg) | R | |||||
| Ceftriaxone (30 µg) | I | |||||
| 59.WK | TS59.WK | pKPT59 | Imipenem (10 µg) | I | blaOXA-48/blaTEM/blaaac(6′)-Ib | 1 plasmid band: >10 kb |
| Ampicillin/sulbactam (20 µg) | R | |||||
| Cefoxitin (30 µg) | R | |||||
| Ceftazidime (30 µg) | R | |||||
| Ceftriaxone (30 µg) | I | |||||
| 66.WK | TS66.WK | pKPT66 | Imipenem (10 µg) | I | blaOXA-48/blaCTX-M | 1 plasmid band: >10 kb |
| Ampicillin/sulbactam (20 µg) | R | |||||
| Cefoxitin (30 µg) | R | |||||
| Ceftazidime (30 µg) | R | |||||
| Ceftriaxone (30 µg) | R | |||||
| Cefepime (30 µg) | SDD | |||||
Notes: blaOXA-48 gene codes for OXA-48-like carbapenemase; blaCTX-M and blaTEM genes code for ESBLs; aac(6ʹ)-Ib gene codes for aminoglycoside 6ʹ-N-acetyltransferase type Ib.