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Pretreatment with sRAGEs alone or in combination with S100A8/A9 differentially regulate the expression of IL-6, IL-1β, TNF-α, and S100A8/A9. Cultured aortic vascular smooth muscle cells were pretreated (1 h) with sRAGEs (50–500 pg/mL) or vehicle (PBS) (A) or in combination with S100A8 or s100A9 (0.05–0.5 g/mL) (B). Bars are mean+SEM of gene mRNA expression. * p < 0.05 vs. respective vehicle; ! p < 0.05 vs. sRAGEs (500 pg/mL). n = 3 different cultures.
