Figure 1.

No enrichment of cancer stem-like cells in FPRMS spheres. (A) Sphere assay with RH4, RH30, and RMS13 cells cultivated in sphere medium supplemented with bFGF (FGF) or with bFGF, EGF, and PDGF (ALL) over indicated number of passages. Percentage of cells forming spheres was calculated at each passage. Represented are means of triplicates (n = 3). (B) Quantification of mRNA expression by real-time PCR of NANOG, OCT4, and SOX2 in spheres of three FPRMS cell lines at passage 3, 4, and 5. Fold increases are calculated compared to the cells cultured as a monolayer. Represented are means of triplicates (n = 3).