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. 2021 Aug 31;12(9):1373. doi: 10.3390/genes12091373

Figure 2.

Figure 2

Lack of subpopulations with cancer stem-like properties in FPRMS cells lines. (A–C) Flow cytometric analysis of CSC markers CD133 (epitope 1 and epitope 2), CD44, and CXCR4 in seven FPRMS cell lines. Percentage of positive cells is indicated. (D) Percentage of ALDH-positive cells after staining with Aldefluor and flow cytometric analysis in FPRMS cell lines. (E,F) Percentage of ALDH-positive cells after staining with Aldefluor in RH4 (E) and RH30 (F). FPRMS cells cultured as a monolayer (cell line), or under sphere conditions, or cells dissociated from xenografts. G, H Distribution of sorted ALDH-positive and -negative fractions in RH4 (G) and RH30 (H) cell lines after indicated days of culture. (I,J) Sphere assay with RH4 and RH30 sorted ALDH-positive, -negative, and control fractions (control fractions were unstained cells processed through the FACS machine and collected without sorting for specific subpopulations). Cells were cultured at clonal density in ALL-sphere medium and primary sphere formation as well as self-renewal ability over 5 passages (p) was quantified. (unpaired t-test: ** p < 0.005, ns: not significant).