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Volume renderings of porcine hair follicles reconstructed from Z-stacks acquired for excitation wavelength of 1100 nm. (A) skin treated with saline solution (Z-step: 1.5 μm, total depth: 222 μm); laser power: 102.4 mW. (B) Skin treated with nile red-loaded TO20 (Z-step: 1 μm, total depth: 222 μm); laser power: 19.2 mW; the detector gain and the laser power were lower for (B) compared to (A) to avoid saturation; for this reason, the SHG of dermis collagen is not visible in panel (B).
