Table 5.
Summary of cellular consequences resulting from interactions between different USPs and target proteins associated with the Wnt signaling pathway.
| USPs | Targets | Cell Model Association | Effects | Refs |
|---|---|---|---|---|
| USP3 | MMP2 | HGC27 cells (gastric carcinoma and xenografts) |
Knockdown of USP3 suppresses proliferation and migration, and promotes G1 cell cycle arrest | [59] |
| Knockdown of USP3 decreases tumor growth in mice bearing tumor xenografts | ||||
| SK-GT-2 cells (gastric adenocarcinoma) |
Overexpression of USP3 leads to increased migration and invasion | |||
| USP4 | β-catenin | HCT116 cells (colorectal carcinoma) |
USP4 knockdown decreases proliferation and invasion | [140] |
| USP5 | β-catenin | A549, H1299 and 95-D (lung adenocarcinoma and xenografts) |
USP5 overexpression increases cellular proliferation in A549 cells | [145] |
| Knockdown of USP5 decreases cellular proliferation in H1299 cells | ||||
| USP5 knockdown decreases tumoral growth in mice bearing tumor xenografts from H1299 cells | ||||
| Knockdown of USP5 reduces β-catenin transcriptional activity and inhibits invasion and migration in H1299 and 95-D cells | [150] | |||
| USP7 | Axin1 | HEK293T cells (kidney) # |
Knockdown of USP7 decreases Axin levels leading to an increase in β-catenin levels and Wnt signaling activation | [151] |
| USP14 | Disheveled (Dvl) | HEK293T cells # (not cancer) (kidney) |
Inhibition of USP14 increases polyubiquitination of Dvl, hindering the progression of Wnt signaling | [142] |
| USP15 | APC | HeLa cells (cervical adenocarcinoma) |
Knockdown of USP15 decreases APC levels and increases β-catenin levels | [152] |
| USP21 | TCF7 | hTERT-HPNE E6/E7 cells (pancreas cancer cells, xenografts) |
USP21 overexpression promotes cellular proliferation and tumoral progression in mice bearing tumor xenografts | [153] |
| USP34 | Axin1 | HEK293T cells (kidney) # |
Knockdown of USP34 decreases Axin1 levels and increases levels of β-catenin in HEK293T cells | [154] |
| USP39 | β-catenin | HO-8910 and SKOV3 (ovarian carcinoma) |
USP39 knockdown decreases β-catenin levels and inhibits cellular migration and invasion | [79] |
| β-catenin, TCF4, MMP2 and MMP9 | HT29 and SW480 cells (colorectal adenocarcinoma) |
USP39 knockdown reduces the expression of β-catenin, TCF4, MMP2 and MMP9, and prevents migration and invasion | [146] | |
| USP44 | Axin1 | HT29 and HCT116 cells (colorectal adenocarcinoma/carcinoma) |
USP4 overexpression increases Axin1 levels and decreases β-catenin, c-Myc and cyclin D1 levels, and also inhibits cellular proliferation and promoting apoptosis | [155] |
#, The cells used were not cancer-derived cells.