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. 2021 Aug 27;13(9):2975. doi: 10.3390/nu13092975

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Assessment of cecal microbiota composition. The sequencing of the amplicon targeting the V3-V4 variable region of the 16S rRNA was performed using the Illumina Miseq sequencing 300x2 approach. The alpha diversity is represented by the richness (a), Pielou’s evenness (b) and Shannon’s indexes (c). The beta diversity was calculated measuring the Unweighted Unifrac distance (d). The taxonomic abundances of the main groups corresponding to family (e) and genera (g) are represented in stacked bars. The qualitative assessment of microbiota is represented in a Venn Diagram at the level of family (f) and genera (h). Results are derived from n = 6 animals/ group. Statistical significance: * p < 0.05 vs. REF, # vs. PRE and ϕ vs. POST. REF: reference group; PRE: group supplemented with a mixture of scGOS and lcFOS; POST: group supplemented with Lactofidus^TM; P+P: group supplemented with the combination of both.