Figure 3.

Nociceptive behavior of trehalose- and placebo-treated mice using the SNI model. Mice were treated continuously with 5% trehalose or placebo via drinking water, starting after surgery. Paw withdrawal latencies were assessed at baseline and up to 6 months after SNI. The scatter plots show individual mice (n = 16 per group), the thick line is the mean. (A): The Hargreaves test shows paw withdrawal latencies of the ipsilateral (left) and contralateral (right) hind paw before and after SNI upon stimulation with radiant heat. (B): Paw withdrawal latencies upon mechanical stimulation in the Dynamic Plantar test. (C): Hotplate latencies at 52 °C of the hotplate surface. (D,E): Cold pain hypersensitivity using a cold plate at 0 °C and in the acetone test. Data were compared with 2-way ANOVA for repeated measurements, using “time” X “treatment” as within- and between-subject factors, and subsequent post hoc analysis with P adjustment according to Šidák. Asterisks indicate statistically significant differences between the treatment groups. * p < 0.05, ** p < 0.01, *** p < 0.001., **** p < 0.0001, n.s. not significant.