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Isolation, culturing and reviving of bovine fungiform taste bud cells. (A) Taste bud cells in 24-well plates after 6 days of seeding. (B) Cells in 24-well plates after 9 days of seeding. (C) Cells in 25-mL flask after 48 hours of seeding. (D) Revived BTBCs following cryopreservation Passage-3 cells; (E) Passage-4 cells and (F) Passage-5 cells.
