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RT-PCR study of mRNA expression of taste cell markers including: (A) α-gustducin (GNAT3), TRPM5, Tas1R1, Tas1R2, PLC-β2 and PKD2L1; (B) Tas2R3 and Snap25; (C) ENTPD2 and T1R3; and (D) voltage-gated sodium channels. (E) mRNA expression of bitter taste receptors; all the gene-specific primers yielded a single PCR product with a correct amplicon size except the second primer set of T2R7; and (F,G) cell fate and differentiation genes.
