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. 2021 Sep 16;10(9):1477. doi: 10.3390/antiox10091477

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Polydatin caused the disturbance of calcium regulation and apoptosis in HCT116 and HT-29 cells. (A) In response to polydatin, the cytosol calcium concentrations in colon cancer cells were evaluated by flow cytometry analysis with Fluo-4 dye. (B) The colon cancer cells treated with polydatin were stained with Rhod-2 dye to analyze mitochondrial calcium concentrations. (C,D) Effects of polydatin with specific calcium-channel inhibitors, 2-APB (IP3R), BAPTA/AM (intracellular calcium), (C) cytosol, and (D) mitochondrial calcium levels in HCT116 and HT-29 cells. (E) Effects of polydatin with each calcium-channel inhibitor on apoptosis in HCT116 and HT-29 cells by Annexin V and PI staining. The asterisks indicate statistical significance of the effect of treatment (* p < 0.05, ** p < 0.01, and *** p < 0.001).