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. 2021 Sep 1;7(9):172. doi: 10.3390/jimaging7090172

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CLSM visualisation (×60 magnification) of different stained Drosophila melanogaster tissues. (a) Drosophila heart tissue following immunofluorescence staining with an antibody against the Atg8a/GABARAP autophagic protein. (b) Different sections of a fly muscle probed with the mitochondrial marker blw/ATP5A (green) in a Parkinson’s disease fly model, showing accumulation of mitochondrial aggregates (arrows) vs. control. In (a,b) nuclei and actin were counterstained with DAPI and phalloidin, respectively. In (a), Z-stacks with a step size of 1 µm were taken using identical settings, and each stack consisted of 26 plane images.

CLSM visualisation (×60 magnification) of different stained Drosophila melanogaster tissues. (a) Drosophila heart tissue following immunofluorescence staining with an antibody against the Atg8a/GABARAP autophagic protein. (b) Different sections of a fly muscle probed with the mitochondrial marker blw/ATP5A (green) in a Parkinson’s disease fly model, showing accumulation of mitochondrial aggregates (arrows) vs. control. In (a,b) nuclei and actin were counterstained with DAPI and phalloidin, respectively. In (a), Z-stacks with a step size of 1 µm were taken using identical settings, and each stack consisted of 26 plane images.