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. 2021 Sep 17;10(9):2458. doi: 10.3390/cells10092458

Figure 1.

Figure 1

Radiotherapy sensitizes NPC cells to killing by NK cells. NPC cells were irradiated with doses from 0 to 6 Gy and cultured thereafter for 24, 48 or 72 h in medium. Cells were then labeled with calcein, plated in a 96-well plate and incubated with NK cells for 4 h at an E:T ratio of 6:1. (A) Lysis of target cells was determined by the measurement of calcein in collected supernatants by an ELISA reader. (B) NK cells were pre-incubated or not with IFNβ (1000 U/mL) for 24 h and then co-incubated with NPC cells irradiated or not with 2 Gy and cultured for 24 h as above. Data are presented as means ± S.E.M. Asterisks indicate statistically significant differences between the non-irradiated group and irradiated groups in all cell lines (a: two-way ANOVA; b: one-way ANOVA; ** p < 0.01; *** p < 0.001).