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. 2021 Sep 17;10(9):2458. doi: 10.3390/cells10092458

Figure 7.

Figure 7

Radiotherapy induces PD-L1 expression in NPC cells via upregulation of NF-κB. C666-1 cells were incubated with the NF-κB inhibitor BMS-345541 for 1 h before radiation. Expression of NF-κB, PD-L1 was analyzed by flow cytometry. (A) Histogram plots of PD-L1 and NF-κB expression on NPC cells; quantification of PD-1 (B) and NF-κB (C) expression on NPC cells. Quantitative data of three independent experiments are shown as normalized mean fluorescence intensity (MFI). Normalized MFI is calculated by dividing the MFI of the stained sample by the MFI of the control antibody. Data is shown as mean ± S.E.M. Asterisks indicate statistically significant differences between all cell lines (two-way ANOVA; *** p < 0.001; n.s.: not significant).