Figure 1.

Analysis of total RNA and RNA modification changes upon chemical stress exposure of S. cerevisiae cultures. (a) Logarithmic growth curve in YNB medium using LD₅₀ concentrations of stressors: H₂O₂ (2 mM), NaAsO₂ (40 mM), HOCl (3 mM), MMS (12 mM), TBH (10 mM). (b) Total RNA of S. cerevisiae isolated with TRI reagent or hot phenol (c) assessed with size exclusion chromatography, 1: 25S, 2: 18S, 3: 5.8S rRNA, 4: tRNA (d) Fold changes in modification density in small RNA < 200 nts: (A) Chan et al. and (B) total tRNA from this work. An increase is shown in red and a decrease in blue. Student’s t-test results: p < 0.05 indicated with *. (e) Fold changes in modification density in 18S and 25S rRNA; Student’s t-test results: p < 0.05 indicated with *. All experiments were done in biological triplicates; error bars reflect standard deviations.