Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Methylome discrimination assay in S. cerevisiae. (a) Titration of optimal L-methionine-[2H3]-methyl supplementation to receive the highest abundance of CD3-labeled RNA modifications. (b–d) Ratios of damaged (red) to enzymatically (black) methylated nucleosides in S. cerevisiae tRNA (b), 25S rRNA (c) and 18S rRNA (d) after 60 min of MMS exposure. (e) The methylome discrimination assay reveals the origin of the methylation in the newly identified RNA damage product m6Am.
