Figure 5.

Incorporation of RNA modifications into new transcripts over time. Cells were initially grown in medium containing ¹⁵N₂-uracil, ¹³C₆-glucose; after stress exposure, the cells were cultivated in medium with L-methionine-[²H₃]-methyl. (a) Abundances of modifications in new tRNA sorted by high and low abundances of modifications. (means of n = 3 and error bars reflect standard deviation). (b,c) Abundance of modifications in new 18S (b) and 25S (c) rRNA. (means of n = 2, and error bars reflect standard deviation). (d) A comparison of modification abundances in new total tRNA with dependence on stress. (means of n = 3, and error bars reflect standard deviations). (e,f) A comparison of modification abundances in new 18S (e) and 25S (f) rRNA in dependence on stress. (means of n = 2, and error bars reflect standard deviations).