Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 4;9(9):1162. doi: 10.3390/biomedicines9091162

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Conditioned medium from IPF-LFs induces senescence in naïve Ctrl-LFs. Sub-confluent naïve recipient Ctrl-LFs were exposed to CM from Ctrl- or IPF-LFs for 3 d (n = 5–10). (a–g) Levels of senescence markers in donor Ctrl- and IPF-LFs prior to culture with CM. Representative immunofluorescence images from Ctrl- and IPF-LF cultures show nuclear localisation of H2AXγ and p21 (blue = DAPI, red = p21 and green = H2AXγ). (h–m) Levels of senescence markers in naïve Ctrl-LFs 3 d after culture with CM. Each data point for the IPF treatment group represents a unique combination of CM from a “donor” IPF patient and recipient Ctrl-LF. CM from LFs of five separate IPF patients were used. (* p < 0.05). Scale bar = 50 μm.