Figure 8.

Nuclear p21 accumulates in naïve fibroblasts co-cultured with senescence-induced fibroblasts. Untreated and CellTrace Far Red dye loaded senescence-induced fibroblasts were seeded together at different ratios and maintained in culture for 1, 5 and 7 d (blue = DAPI, green = p21, red = senescence-induced fibroblasts). (a) Monocultures of untreated fibroblasts (100:0). (b) Monocultures of senescence-induced fibroblasts (0:100). (c–e) Co-cultures established from different seeding ratios of untreated and senescence-induced cells (50:50, 90:10 and 99:1). Scale bar = 50 µm. (f) The percentage area of nucleus associated with p21 in untreated naive fibroblasts after co-culture with senescence-induced fibroblasts for 7 d (n = 3).