Figure 9.

Nuclear H2AXγ increases in untreated naïve fibroblasts co-cultured with senescence-induced fibroblasts. Untreated and fluorescent-labelled senescence-induced fibroblasts were co-cultured at different ratios and maintained in culture for 1, 5 and 7 d (blue = DAPI, green = H2AXγ, red = senescence-induced fibroblasts). (a,b) Monocultures of untreated (100:0) and senescence-induced fibroblasts (0:100). (c–e) Co-cultures of untreated and senescence-induced fibroblasts at different ratios (50:50, 90:10 and 99:1). Scale bar = 50 µm. (f) The percentage area of nucleus associated with H2AXγ in non-treated naïve fibroblasts after co-culture with senescence-induced fibroblasts for 7 d (n = 3).