FIG. 1.

The NES of Net. (A) Alignment of NES sequences. The NES-like sequence in the ets domain of Net (residues 6 to 18 in mice and humans) is aligned with known NESes. The functionally important hydrophobic residues are shown in boldface. Sequences used for comparison: MAPKK (10), cAMP-dependent protein kinase inhibitor (PKI) (79), HIV-1 Rev (7), Dsk-1 (9), and cyclin B1 (73). The Net NES mutants mP (F10K, L11P, L12P, H13V, L14P, L15P, L16S), mA1 (L11A, L12A), and mA2 (L14A, L15A, L16A) have the amino acids changes shown in parentheses. (B) Cellular localization of GFP, GFP-Net/NES, and GFP-Net/NES mP. Drawings representing GFP fused to NES wild-type (WT) and mP sequences are shown. NIH 3T3 cells transiently expressing GFP, GFP-Net/Nes, and GFP-Net/NES mP, were analyzed by confocal microscopy. Hoechst was used to stain nuclei.