FIG. 4.

NES-like sequences in other ets domains do not mediate nuclear export. (A) Comparison of mouse and human Net (mNet and hNet) NES with corresponding sequences in other Ets family members. The Ets proteins used for the alignment are human Elk1, human Sap1a, mouse Pea3, mouse Ets1, human Erf, human Elf1, Drosophila Yan (Pok), and human Pu1. Cons., consensus. Cellular localization of the corresponding GFP-NES fusion proteins is summarized on the right. N, nuclear; C, cytoplasmic; N/C, both nuclear and cytoplasmic. (B) Cellular localization of GFP fusion proteins. Proteins with GFP linked to the sequences listed in panel A were transiently expressed in NIH 3T3 cells and localized by confocal microscopy. Only GFP-Net/NES and GFP-Elk1 are shown. The localization of the other GFP-Ets proteins was similar to that of GFP-Elk1, as summarized in panel A.