TABLE 2.
Disruption of NES activitya
| NES | Sequence | Localization
|
||
|---|---|---|---|---|
| N | C | N/C | ||
| Wild-type NES | TLWQFLLHLLLDQ | − | + | − |
| NES (L16R) | TLWQFLLHLLRDQ | − | − | + |
| NES (L16A) | TLWQFLLHLLADQ | − | − | + |
| NES (L16P) | TLWQFLLHLLPDQ | − | − | + |
| NES (D17E) | TLWQFLLHLLLEQ | − | + | − |
| NES consensus | XLXXXLXXLXLXX | |||
| Ets consensus | XLXXXLLXLLXXX | |||
a
The Net NES mutations (underlined) were leucine 16 to either arginine [Net(L16R)], alanine [Net(L16A)] or proline [Net(L16P)] and aspartate 17 to glutamate [Net(D17E)]. Wild-type and mutant NESs were fused to GFP, and the cellular localization of each fusion protein was determined. N, nuclear; C, cytoplasmic; N/C, both nuclear and cytoplasmic.