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. 2021 Sep 9;22(18):9746. doi: 10.3390/ijms22189746

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of DHT and diet on renal SARS-CoV-2 viral entry proteins expression. Animals were treated with dihydrotestosterone (DHT) or vehicle (Veh) and maintained in low (LFD) or high (HFD) fat diet for 90 days. Ace2 (A), Tmprss2 (B), Tmprss4 (C), furin (D), cathepsin L (E), and ADAM17 (F) mRNA was quantified by RT-qPCR and standardized to the geometric mean of four housekeeping genes (HGMK) (N = 6–8/group). ACE2 (G), TMPRSS2 (H), TMPRSS4 (I), furin (J), cathepsin L (K), and ADAM17 (L) protein was quantified by Western-blot and normalized to GAPDH (N = 4/group). Total furin (J) was quantified as the sum of profurin (dashed arrow) and cleaved soluble furin (solid arrow). Data are expressed as mean ± SEM. Data were analyzed by two-way ANOVA followed by Fisher’s LSD test. ^a p < 0.05 vs. LFD-Veh; ^b p < 0.05 vs. LFD-Veh; ^c p < 0.05 vs. LFD-DHT.