Figure 4.

Metabolism of LA, 1-LG or LEA by human eosinophils. Pre-warmed human eosinophil suspensions (37 °C, 10⁶ cells/mL in HBSS containing 1.6 mM CaCl₂) were treated with DMSO (A–C) or MAFP 1 μM (E–G) for 5 min then stimulated with 3 μM of (A,E) 1-LG, (B,F) LEA, (C,G) LA for another 5 min. (D) Metabolite legend for panels. (A–G) Incubations were stopped by the addition of one volume of cold (−20 °C) MeOH containing the internal standards. Samples then were processed for LC-MS analysis as described in methods. Results are the mean (±SEM) of 4–5 independent experiments.