Figure 6.

15-LO metabolite production in response to LA, 1-LG, or LEA by human neutrophils. Pre-warmed human neutrophil suspensions (37 °C, 5 × 10⁶ cells/mL in HBSS containing 1.6 mM CaCl₂) were pre-treated with DMSO (A–C) or MAFP (E–G) for 5 min before the addition of 3 μM of either (A,E) 1-LG, (B,F) LEA, (C,G) LA. (D) Metabolite legend for panels A–G. Incubations were stopped after 5 min by the addition of one volume of cold (−20 °C) MeOH containing the internal standards. Samples then were processed for LC-MS/MS analyses as described in methods. Results are the mean (±SEM) of 4 independent experiments.